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Search probeBase

Probes/primers can be found by searching for the name of the target organism or taxon, the name of the oligonucleotide itself, the reference that originally described the oligonucleotide, the sequence of the oligonucleotide or the specific target sites on the rRNA molecule. Help
Search target organisms
The specificity entry of each probe/primer indicates the intended target organism(s) as described in the original publication or during user submissions to probeBase. Furthermore, each oligonucleotide in probeBase is assigned to a taxon in the NCBI taxonomy. Please note that this taxonomic assignment does not necessarily mean that an oligonucleotide is highly specific for a given taxon, but it allows for more advanced searches. A search for a target organism or taxon queries the specificity and taxonomy entries and systematically returns all available oligonucleotides. The search can be further adjusted by including oligonucleotides that target higher and/or lower taxonomic ranks than the query taxon. The output list of probes that target the query organism at different taxonomic levels helps researchers identifying sets of hierarchically nested probes for application in multiple-probe hybridization formats such as multi-color FISH and DNA microarrays. Help
Search probe/primer names and papers
Search probe/primer sequence
Search target sites
Use this search function to identify probes/primers that bind in a defined region of the rRNA molecule by entering the binding position according to the E. coli numbering (e.g. enter 338 to find all oligonucleotides that bind near the target position of probe EUB338). This tool further assists in the development of new oligonucleotide probes for FISH by providing information on whether a searched target site has previously been found accessible for oligonucleotide probes in other microorganisms. Help