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Reference Molecular identification of an uncultured bacterium ("morphotype R") in meromictic Lake Cadagno, Switzerland. Tonolla M, Bottinelli M, Demarta A, Peduzzi R, Hahn D. FEMS microbiology ecology. 2005.
Abstract Comparative sequence analysis of almost complete 16S rRNA genes of members of the Desulfobacteriaceae retrieved from two gene clone libraries of uncultured bacteria of the chemocline of Lake Cadagno, Switzerland, resulted in the molecular identification of nine sequences, with a tight cluster of five sequences that represented at least three different populations of bacteria with homology values of 95% and 93% to their closest cultured relatives Desulfomonile tiedjei and Desulfomonile limimaris, respectively. In situ hybridization with probes DsmA455 targeting two subpopulations and DsmB455 targeting one subpopulation, detected bacteria with a peculiar morphology previously described as "morphotype R". The individual probes detected about the same number of cells in all samples and together added up to represent all cells of "morphotype R" suggesting that the basic ecophysiological requirements of the subpopulations might be similar. In the monimolimnion, "morphotype R" cells accounted for up to 29% of all Bacteria and entirely represented the Desulfobacteriaceae, the most prominent sulfate-reducing bacteria. In the sediment, "morphotype R" was similarly prominent in the upper cm only where it represented all Desulfobacteriaceae and up to 50% of all Bacteria. Numbers and importance within the Desulfobacteriaceae and Bacteria declined significantly with depth in sediments suggesting potential effects of changing environmental conditions on the fate of "morphotype R".
Pubmed ID 16329943
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