Calculating ...

Press ESC to cancel.

Probe/primer details

1061R To be used as primer!
Full name
(Alm et al. 1996)
S-D-Bact-1061-a-A-19
Accession no. pB-3807
Taxonomy Bacteria
Specificity Bacteria
Category primer
Target rRNA 16S rRNA
Direction reverse primer
Position 1061-1079
Sequence 5'- CAC GRC ACG AGC TGA CGA C -3'
G+C content [%] 63
Length [nt] 19
Check specificity/coverage
Evaluate primer pair
(service provided by silva)
Tm 56.5
Hybridization efficiency
References

Illumina-based analysis of microbial community diversity. Degnan PH, Ochman H. The ISME journal. 2012. Pubmed

Print

Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.