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Probe/primer details

U529R To be used as primer!
Full name
(Alm et al. 1996)
S-*-Univ-0522-a-A-18
Accession no. pB-3743
Taxonomy cellular organisms
Specificity Bacteria, Archaea, Eukarya
Category primer
Target rRNA 16S rRNA
Direction reverse primer
Position 522-536
Sequence 5'- ACC GCG GCK GCT GGC -3'
G+C content [%] 80
Length [nt] 15
Check specificity/coverage
Evaluate primer pair
(service provided by silva)
Tm 54.5
Hybridization efficiency
References

Review and re-analysis of domain-specific 16S primers. Baker GC, Smith JJ, Cowan DA. Journal of microbiological methods. 2003. Pubmed

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.