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Probe/primer details

Name CC23a
Accession no. pB-333
Taxonomy Caedibacter caryophilus; Caedibacter; Rickettsiales genera incertae sedis; Rickettsiales; Alphaproteobacteria; Proteobacteria; Bacteria
Specificity Caedibacter caryophilus (C. caryophyla) and other Caedibacter-related endosymbionts of Acanthamoeba spp.
Category symbiotic microbes
Target rRNA 16S rRNA
Position 658-675
Sequence 5'- TTC CAC TTT CCT CTC TCG -3'
G+C content [%] 50
Length [nt] 18
Check specificity/coverage
Hybridization efficiency
References

Occurrence of fragmented 16S rRNA in an obligate bacterial endosymbiont of Paramecium caudatum. Springer N, Ludwig W, Amann R, Schmidt HJ, Görtz HD, Schleifer KH. Proceedings of the National Academy of Sciences of the United States of America. 1993. Pubmed

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.