Probe/primer details
| Name | Mcell-181(AcidM-181) |
| Accession no. | pB-209 |
| Taxonomy | Beijerinckiaceae; Rhizobiales; Alphaproteobacteria; Proteobacteria; Bacteria |
| Specificity | Methylocella palustris+Methylocapsa acidiphila |
| Category | methylotrophic bacteria |
| Target rRNA | 16S rRNA |
| Position | 181-198 |
| Sequence | 5'- TCT TTC TCC TTG CGG ACG -3' |
| G+C content [%] | 56 |
| Length [nt] | 18 |
| Check specificity/coverage | 
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| Hybridization efficiency |  |
| References | Detection and enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA fluorescence in situ hybridization, including the use of newly developed oligonucleotide probes for Methylocella palustris. Dedysh SN, Derakshani M, Liesack W. Applied and environmental microbiology. 2001. Pubmed Differential detection of type II methanotrophic bacteria in acidic peatlands using newly developed 16S rRNA-targeted fluorescent oligonucleotide probes. Dedysh SN, Dunfield PF, Derakshani M, Stubner S, Heyer J, Liesack W. FEMS microbiology ecology. 2003. Pubmed |
| Remarks | helper probe H158 |
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.
