Probe/primer details
| Name | Eco09i |
| Accession no. | pB-114 |
| Taxonomy | Enterococcus columbae; Enterococcus; Enterococcaceae; Lactobacillales; Bacilli; Firmicutes; Bacteria |
| Specificity | Enterococcus columbae |
| Category | bacteria of medical or hygienical relevance |
| Target rRNA | 23S rRNA |
| Position | 142-159 |
| Sequence | 5'- GGA TAT TAC CTT TAA GTG -3' |
| G+C content [%] | 33 |
| Length [nt] | 18 |
| Check specificity/coverage | 
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| Hybridization efficiency |  |
| References | A nested array of rRNA targeted probes for the detection and identification of enterococci by reverse hybridization. Behr T, Koob C, Schedl M, Mehlen A, Meier H, Knopp D, Frahm E, Obst U, Schleifer K, Niessner R, Ludwig W. Systematic and applied microbiology. 2000. Pubmed |
| Remarks | Suffix “i” in the probe name indicates that this probe can not be used to detect 23S rRNA. The reverse complementary version of this probe targets 23S rRNA. |
| Used in Microarray | ECC-PhyloChip |
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.
