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Probe/primer details

Lbrev Tested for in situ hybridization.
Accession no. pB-1006
Taxonomy Lactobacillus brevis; Lactobacillus; Lactobacillaceae; Lactobacillales; Bacilli; Firmicutes; Bacteria
Specificity Lactobacillus brevis
Target rRNA 16S rRNA
Position 64-83
Sequence 5'- CAT TCA ACG GAA GCT CGT TC -3'
G+C content [%] 50
Length [nt] 20
Check specificity/coverage
Hybridization efficiency
References

Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. Blasco L, Ferrer S, Pardo I. FEMS microbiology letters. 2003. Pubmed

Remarks cell treatment with 10 µl of 10 mg lysozyme per ml in 100 mM Tris-HCl and 50 mM EDTA (pH 8.0) for 20 min at 4°C or 5 min at room temperature

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.