Microarray details
| Array name | Nitrifier-Microarray |
| Array Description | Gel-based oligonucleotide microarray (microchip) for detection of nitrifying bacteria. The microarray contains 26 16S rRNA-targeted oligonucleotide probes at varying levels of specificity. |
| Array technique | Oligonucleotide probes were immobilized through reductive coupling of the 3′ amino group of the oligonucleotide with the aldehyde group of the activated gel pad on the microarrays |
| Reference | Impact of prehybridization PCR amplification on microarray detection of nitrifying bacteria in wastewater treatment plant samples. Siripong S, Kelly JJ, Stahl DA, Rittmann BE. Environmental microbiology. 2006. |
| Probename | Name (Alm et al.) |
|---|---|
| EUB338 (Bact338) (bacterial) | S-D-Bact-0338-a-A-18 |
| NB1000 | S-G-Nbac-1000-a-A-15 |
| NIT3 | S-G-Nbac-1035-a-A-18 |
| Nse1472 | S-*-Nse-1472-a-A-18 |
| Nsm156 | S-G-Nsm-0155-a-A-19 |
| Nso1225 | S-F-bAOB-1224-a-A-20 |
| Nso190 | S-F-bAOB-0189-a-A-19 |
| Nsv443 | |
| Ntspa662 | S-G-Ntspa-662-a-A-18 |
| Ntspa712 | S-*-Ntspa-712-a-A-21 |
| UNIV1390 | S-*-Univ-1390-a-A-18 |
| NEU | S-*-Nsm-0651-a-A-18 |
| NSR826 | |
| NSR1156 | |
| Nmo218 | |
| Univ907 | S-*-Univ-0907-a-A-18 |
| Eub927 | S-D-Eub-0927-a-A-18 |
| NmII | S-S-Nsom-0120-a-A-20 |
| NmIII | S-S-Nsom-0998-a-A-21 |
| NmIV | S-S-Nsom-1004-a-A-19 |
| Nsr1145 | S-*-Nsr-1145-a-A-18 |
| Nsc825 | S-*-Nsc-0825-a-A-18 |
| Nsr989 | S-S-Nsr-0989-a-A-18 |
| Nsn227 | S-*-Nsn-0227-a-A-18 |
| Ncc214 | S-S-Ncc-0214-a-A-18 |
Color codes
- Primer
- Probes used for FISH
