Calculating ...

Press ESC to cancel.

Microarray details

Array name Fish Pathogen 16S Microarray
Array Description Oligonucleotide-based microarray used to simultaneously screen for 15 fish pathogens.
Array technique icroarray probes (22- to 31-mer oligonucleotides) were spotted onto Teflon-masked, epoxy-silane-derivatized glass slides using a robotic arrayer. PCR products (ca. 199 bp) were generated using biotinylated, universal primer sequences, and these products were hybridized overnight (55 degrees C) to the microarray. Targets that annealed to microarray probes were detected using a combination of Tyramide Signal Amplification and Alexa Fluor 546.
Reference Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays. Warsen AE, Krug MJ, LaFrentz S, Stanek DR, Loge FJ, Call DR. Applied and environmental microbiology. 2004.
<< >>
  • Color codes

    • Primer
    • Probes used for FISH