| Name | Lpla67 |
| Accession no. | pB-3941 |
| Taxonomy | Lactobacillus plantarum [1]; Lactobacillus [1]; Lactobacillaceae [1]; Lactobacillales [1]; Bacilli [1]; Firmicutes [1]; Bacteria [1] |
| Specificity | Lactobacillus plantarum |
| Category | animal and human associated microbiota [1] intestinal microbiota [1] |
| Target rRNA | 16S rRNA |
| Position | 67-86 |
| Sequence | 5'- CCA ATC AAT ACC AGA GTT CG -3' |
| G+C content [%] | 45 |
| Length [nt] | 20 |
| Check specificity/coverage |  [2]
|
| Hybridization efficiency |  [2] |
| References | Molecular approaches for the detection and identification of bifidobacteria and lactobacilli in the human gastrointestinal tract. Satokari RM, Vaughan EE, Smidt H, Saarela M, Mättö J, de Vos WM. Systematic and applied microbiology. 2003. Pubmed [2] Hensiek, R., Krupp, G., and Stackebrandt, E.: Development of diagnostic oligonucleotide probes for four Lactobacillus species occurring in the intestinal tract. System. Appl. Microbiol. 15, 123–128 (1992). |
Print [1]
Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED) [2].
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt [2].
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text [2]
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.