probeBase 2016
Published on probeBase 2016 (https://probebase.csb.univie.ac.at)

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Probe/primer details

fir5-575 Tested for in situ hybridization.
Accession no. pB-2684
Taxonomy Firmicutes [1]; Bacteria [1]
Specificity Firm-5 (symbiotic Firmicutes phylotype in honey bee)
Category animal and human associated microbiota [1]
intestinal microbiota [1]
Target rRNA 16S rRNA
Position 575-589
Sequence 5'- TCC CGC CTG CGT TCG -3'
G+C content [%] 73
Length [nt] 15
Check specificity/coverage [2]
Hybridization efficiency [2]
References

Establishment of characteristic gut bacteria during development of the honeybee worker. Martinson VG, Moy J, Moran NA. Applied and environmental microbiology. 2012. Pubmed [2]

Print [1]

Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED) [2].
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt [2].
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text [2]
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.

probeBase by Daniel Greuter, Alexander Loy, Matthias Horn, and Thomas Rattei, Department of Microbiology and Ecosystem Science, University of Vienna
Questions, comments, bug reports, and suggestions are welcome at probebase@microbial-ecology.net
Greuter et al. 2016. Nucleic Acids Res. 10.1093/nar/gkv1232

Imprint

Source URL:https://probebase.csb.univie.ac.at/pb_report/probe/2684

Links
[1] https://probebase.csb.univie.ac.at/https [2] https://probebase.csb.univie.ac.at/http