Paper details
| Reference | Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms. Amann RI, Stromley J, Devereux R, Key R, Stahl DA. Applied and environmental microbiology. 1992. |
| Abstract | The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms. |
| Pubmed ID | 1376982 |
