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Probe/primer details

DSF672 Tested for in situ hybridization.
Accession no. pB-80
Taxonomy Desulfobacteraceae; Desulfobacterales; Deltaproteobacteria; delta/epsilon subdivisions; Proteobacteria; Bacteria
Specificity Desulfofrigus, Desulfofaba gelida, Desulfomusa hansenii
Category sulfate-reducing microbes
Target rRNA 16S rRNA
Modified version(s) DSB674
Position 672-690
Sequence 5'- CCT CTA CAC CTG GAA TTC C -3'
G+C content [%] 53
Length [nt] 19
Check specificity/coverage
Formamide [%] 45
Hybridization efficiency

Community structure, cellular rRNA content, and activity of sulfate-reducing bacteria in marine arctic sediments. Ravenschlag K, Sahm K, Knoblauch C, Jørgensen BB, Amann R. Applied and environmental microbiology. 2000. Pubmed

Diversity and vertical distribution of cultured and uncultured Deltaproteobacteria in an intertidal mud flat of the Wadden Sea. Mussmann M, Ishii K, Rabus R, Amann R. Environmental microbiology. 2005. Pubmed

Remarks 70 % FA for CARD-FISH


Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.