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Probe/primer details

BKE Tested for in situ hybridization.
Accession no. pB-367
Taxonomy Brevibacterium; Brevibacteriaceae; Micrococcales; Actinobacteria; Actinobacteria; Bacteria
Specificity Brevibacterium sp. (B. ketoglutamicum)
Target rRNA 23S rRNA
Position 12-30
Sequence 5'- GGT TAG CAT CAC TGA TTC A -3'
G+C content [%] 42
Length [nt] 19
Check specificity/coverage
Hybridization efficiency

In situ probing of gram-positive bacteria with high DNA G + C content using 23S rRNA-targeted oligonucleotides. Roller C, Wagner M, Amann R, Ludwig W, Schleifer KH. Microbiology (Reading, England). 1994. Pubmed

Remarks Erratum in: Microbiol. (1995) 141: 1267


Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.