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Probe/primer details

DEN67 Tested for in situ hybridization.
Accession no. pB-2563
Taxonomy Methylophilaceae; Methylophilales; Betaproteobacteria; Proteobacteria; Bacteria
Specificity Methanol-utilizing denitrifying cluster group A closely related to the obligate methylotrophs Methylobacillus and Methylophilus in the order Methylophilales
Target rRNA 16S rRNA
Position 67-84
Sequence 5'- CAA GCA CCC GCG CTG CCG -3'
G+C content [%] 78
Length [nt] 18
Check specificity/coverage
Formamide [%] 35
Hybridization efficiency
References

Use of stable-isotope probing, full-cycle rRNA analysis, and fluorescence in situ hybridization-microautoradiography to study a methanol-fed denitrifying microbial community. Ginige MP, Hugenholtz P, Daims H, Wagner M, Keller J, Blackall LL. Applied and environmental microbiology. 2004. Pubmed

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.