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Probe/primer details

FL197 Tested for in situ hybridization.
Accession no. pB-2533
Taxonomy Flavobacteriia; Bacteroidetes; Bacteroidetes/Chlorobi group; Bacteria
Specificity marine uncultured Flavobacteria clade VIS4
Competitor 5'-TCA TCC CCT ACC GTA ACC -3'
Target rRNA 16S rRNA
Position 197-214
Sequence 5'- TCA TCT CAT ACC GTA ACC -3'
G+C content [%] 44
Length [nt] 18
Check specificity/coverage
Formamide [%] 35
Hybridization efficiency

Distinct flavobacterial communities in contrasting water masses of the north Atlantic Ocean. Gómez-Pereira PR, Fuchs BM, Alonso C, Oliver MJ, van Beusekom JE, Amann R. The ISME journal. 2010. Pubmed


Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.