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Probe/primer details

Name MICR#2
Accession no. pB-213
Taxonomy Microcystis; Chroococcales; Oscillatoriophycideae; Cyanobacteria; Bacteria
Specificity Microcystis aeruginosa NIVA-CYA 143, 57, 228/1, 43, 123/1, 31, 166, M. cf. ichthyoblabe, NIVA-CYA 279, M. cf. wesenbergii NIVA-CYA 172/5, M. viridis NIVA-CYA 122/2
Target rRNA 16S rRNA
Position 580-601
Sequence 5'- GCA GGT GGT CAG CCA AGT CTG C -3'
G+C content [%] 64
Length [nt] 22
Check specificity/coverage
Hybridization efficiency

Application of sequence-specific labeled 16S rRNA gene oligonucleotide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization. Rudi K, Skulberg OM, Skulberg R, Jakobsen KS. Applied and environmental microbiology. 2000. Pubmed


Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.