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Probe/primer details

Name KO 225
Full name
(Alm et al. 1996)
S-S-Cperf-0185-a-A-20
Accession no. pB-1289
Taxonomy Clostridium perfringens; Clostridium; Clostridiaceae; Clostridiales; Clostridia; Firmicutes; Bacteria
Specificity Clostridium perfringens
Target rRNA 16S rRNA
Position 185-204
Sequence 5'- CCT TTG GTT GAA TGA TGA TG -3'
G+C content [%] 40
Length [nt] 20
Check specificity/coverage
Hybridization efficiency
References

Design and application of an oligonucleotide microarray for the investigation of compost microbial communities. Franke-Whittle IH, Klammer SH, Insam H. Journal of microbiological methods. 2005. Pubmed

Used in Microarray Compost Community-Microarray

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.