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Probe/primer details

Name Nsr1145
Full name
(Alm et al. 1996)
S-*-Nsr-1145-a-A-18
Accession no. pB-1240
Taxonomy Nitrospira; Nitrospiraceae; Nitrospirales; Nitrospira; Nitrospirae; Bacteria
Specificity Nitrospira spp.
Category nitrite-oxidizing bacteria
Target rRNA 16S rRNA
Position 1145-1162
Sequence 5'- GGC AGT CCT TTC AGA GTG -3'
G+C content [%] 56
Length [nt] 18
Check specificity/coverage
Hybridization efficiency
References

Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant. Harms G, Layton AC, Dionisi HM, Gregory IR, Garrett VM, Hawkins SA, Robinson KG, Sayler GS. Environmental science & technology. 2003. Pubmed

Impact of prehybridization PCR amplification on microarray detection of nitrifying bacteria in wastewater treatment plant samples. Siripong S, Kelly JJ, Stahl DA, Rittmann BE. Environmental microbiology. 2006. Pubmed

Used in Microarray Nitrifier-Microarray

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Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.