Calculating ...

Press ESC to cancel.

Probe/primer details

MX825c Tested for in situ hybridization.
Accession no. pB-1150
Taxonomy Methanosaetaceae; Methanosarcinales; Methanomicrobia; Euryarchaeota; Archaea
Specificity Methanosaetaceae subgroup
Category methanogenic archaea
Target rRNA 16S rRNA
Position 825-847
Sequence 5'- TCG CAC CGT GGC TGA CAC CTA GC -3'
G+C content [%] 65
Length [nt] 23
Check specificity/coverage
Formamide [%] 50
Hybridization efficiency
References

An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH). Crocetti G, Murto M, Björnsson L. Journal of microbiological methods. 2006. Pubmed

Print

Glossary
Name (Alm et al., 1996). Probe designation according to Alm, E. W., Oerther, D. B., Larsen, N., Stahl, D. A., Raskin, L. (1996). The oligonucleotide probe database. Appl Environ Microbiol 62: 3557-9. Abstract (PUBMED).
Position. Probe position according to the E. coli gene numbering.
Sequence. Sequence in IUPAC code: R=G/A, Y=T/C, M=A/C, K=G/T, S=G/C, W=A/T, H=A/C/T, B=G/T/C, V=G/C/A, D=G/A/T, N=G/A/T/C
Tm. Dissoziation temperature according to: Tm=64.9 + 41 x ((G + C - 16.4)/length).
Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.
Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments.
Coverage. Coverage of the three domains calculated using the SILVA reference database 106 if no or a single mismatch is allowed. The detailed method is described in Klindworth et al., 2012. Nucleic Acids Res. 10.1093/nar/gks808 Full Text
Check specificity/coverage. Use these options to reveal the in silico specificity (i.e. number of matching rRNA sequences outside the target taxon) and coverage (i.e. percentage of matching rRNA sequences within the target taxon) of an oligonucleotide against the most recent SSU and LSU rRNA sequence databases.